Stanford Human Immune Monitoring Facility (IMF)
The Stanford Human Immune Monitoring Facility within Stanford's Institute of Immunology, Transplantation and Infectious Disease provides Stanford researchers access to a battery of cutting-edge cellular, proteomic and genomic assays to study biological samples derived from patients with cancer, autoimmunity and infectious diseases and complications arising from tissue transplantation. These services play a vital role in the work of the Program in Cancer Immunology and Immunotherapy. The facility is directed by William Robinson, MD, PhD.
Basic assays include:
- flow cytometry analysis of lymphocyte populations
- transcriptional profiling of RNA isolated from PBMCs
- multiplex cytokine analysis
Custom assays include:
- phospho-flow cytometry analysis of signaling cascades
- lysate array analysis of signaling molecules
- antigen array analysis of antibody responses
- MHC:peptide tetramer arrays to detect and functionally analyze antigen-specific T cells
- quantitative PCR analysis of mRNA

