Cancer Institute A national cancer institute
designated cancer center

Atul Butte

Publication Details

  • Comparison of multiplex meta analysis techniques for understanding the acute rejection of solid organ transplants Morgan, A. A., Khatri, P., Jones, R. H., Sarwal, M. M., Butte, A. J. BIOMED CENTRAL LTD. 2010

    Abstract:

    Combining the results of studies using highly parallelized measurements of gene expression such as microarrays and RNAseq offer unique challenges in meta analysis. Motivated by a need for a deeper understanding of organ transplant rejection, we combine the data from five separate studies to compare acute rejection versus stability after solid organ transplantation, and use this data to examine approaches to multiplex meta analysis.We demonstrate that a commonly used parametric effect size estimate approach and a commonly used non-parametric method give very different results in prioritizing genes. The parametric method providing a meta effect estimate was superior at ranking genes based on our gold-standard of identifying immune response genes in the transplant rejection datasets.Different methods of multiplex analysis can give substantially different results. The method which is best for any given application will likely depend on the particular domain, and it remains for future work to see if any one method is consistently better at identifying important biological signal across gene expression experiments.

    View details for DOI 10.1186/1471-2105-11-S9-S6

    View details for Web of Science ID 000290218700006

    View details for PubMedID 21044364

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