Cancer Institute A national cancer institute
designated cancer center

Christopher H. Contag

Publication Details

  • Micromirror-scanned dual-axis confocal microscope utilizing a gradient-index relay lens for image guidance during brain surgery JOURNAL OF BIOMEDICAL OPTICS Liu, J. T., Mandella, M. J., Loewke, N. O., Haeberle, H., Ra, H., Piyawattanametha, W., Solgaard, O., Kino, G. S., Contag, C. H. 2010; 15 (2)


    A fluorescence confocal microscope incorporating a 1.8-mm-diam gradient-index relay lens is developed for in vivo histological guidance during resection of brain tumors. The microscope utilizes a dual-axis confocal architecture to efficiently reject out-of-focus light for high-contrast optical sectioning. A biaxial microelectromechanical system (MEMS) scanning mirror is actuated at resonance along each axis to achieve a large field of view with low-voltage waveforms. The unstable Lissajous scan, which results from actuating the orthogonal axes of the MEMS mirror at highly disparate resonance frequencies, is optimized to fully sample 500x500 pixels at two frames per second. Optically sectioned fluorescence images of brain tissues are obtained in living mice to demonstrate the utility of this microscope for image-guided resections.

    View details for DOI 10.1117/1.3386055

    View details for Web of Science ID 000278465300060

    View details for PubMedID 20459274

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