Cancer Institute A national cancer institute
designated cancer center

Matthew Bogyo

Publication Details

  • Cathepsin X-mediated beta(2) integrin activation results in nanotube outgrowth CELLULAR AND MOLECULAR LIFE SCIENCES Obermajer, N., Jevnikar, Z., Doljak, B., Sadaghiani, A. M., Bogyo, M., Kos, J. 2009; 66 (6): 1126-1134

    Abstract:

    Membrane nanotubes were recently described as a new principle of cell-cell communication enabling complex and specific messaging to distant cells. Calcium fluxes, vesicles, and cell-surface components can all traffic between cells connected by nanotubes. Here we report for the first time the mechanism of membrane nanotube formation in T cells through LFA-1 (CD11a/CD18; alpha(L)beta(2)) integrin activation by the cysteine protease cathepsin X. Cathepsin X is shown to induce persistent LFA-1 activation. Cathepsin X-upregulated T cells exhibit increased homotypic aggregation and polarized, migration-associated morphology in 2D and 3D models, respectively. In these cells, extended uropods are frequently formed, which subsequently elongate to nanotubes connecting T lymphocytes. Our results demonstrate that LFA-1 activation with subsequent cytoskeletal reorganization induces signal transmission through a physically connected network of T lymphocytes for better coordination of their action at various stages of the immune response.

    View details for DOI 10.1007/s00018-009-8829-8

    View details for Web of Science ID 000264174800014

    View details for PubMedID 19194656

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