Cancer Institute A national cancer institute
designated cancer center

Dean W. Felsher

Publication Details

  • An efficient and versatile system for acute and chronic modulation of renal tubular function in transgenic mice NATURE MEDICINE Traykova-Brauch, M., Schoenig, K., Greiner, O., Miloud, T., Jauch, A., Bode, M., Felsher, D. W., Glick, A. B., Kwiatkowski, D. J., Bujard, H., Horst, J., Doeberitz, M. v., Niggli, F. K., Kriz, W., Groene, H., Koesters, R. 2008; 14 (9): 979-984


    We describe a transgenic mouse line, Pax8-rtTA, which, under control of the mouse Pax8 promoter, directs high levels of expression of the reverse tetracycline-dependent transactivator (rtTA) to all proximal and distal tubules and the entire collecting duct system of both embryonic and adult kidneys. Using crosses of Pax8-rtTA mice with tetracycline-responsive c-MYC mice, we established a new, inducible model of polycystic kidney disease that can mimic adult onset and that shows progression to renal malignant disease. When targeting the expression of transforming growth factor beta-1 to the kidney, we avoided early lethality by discontinuous treatment and successfully established an inducible model of renal fibrosis. Finally, a conditional knockout of the gene encoding tuberous sclerosis complex-1 was achieved, which resulted in the early outgrowth of giant polycystic kidneys reminiscent of autosomal recessive polycystic kidney disease. These experiments establish Pax8-rtTA mice as a powerful tool for modeling renal diseases in transgenic mice.

    View details for DOI 10.1038/nm.1865

    View details for Web of Science ID 000258988600032

    View details for PubMedID 18724376

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