Cancer Institute A national cancer institute
designated cancer center

Christopher H. Contag

Publication Details

  • HIV type 1 envelope subtype C sequences from recent seroconverters in Zimbabwe AIDS RESEARCH AND HUMAN RETROVIRUSES Batra, M., Tien, P. C., Shafer, R. W., Contag, C. H., Katzenstein, D. A. 2000; 16 (10): 973-979

    Abstract:

    HIV-1 envelope sequence patterns have implications for virus cell tropism and for the development of an effective vaccine. To identify the sequence characteristics of recently transmitted HIV-1 isolates in southern Africa, we sequenced the V3-V5 envelope regions of 24 male seroconverters in Harare, Zimbabwe. Each of the sequences clustered with previously reported subtype C isolates and there was a mean 17% intersequence pairwise genetic distance between the Zimbabwean isolates. Three isolates were syncytium inducing (SI). One of the SI isolates had an unusual GIGK crown and a deletion at codon 23; one had the codon 23 deletion alone; and one had a high net positive charge in the V3 loop. The extensive genetic diversity within the envelope of subtype C HIV-1 isolates must be considered in vaccine development. Further analysis of subtype C SI isolates and site-directed mutagenesis experiments are required to determine the molecular basis of SI activity in global HIV-1 isolates.

    View details for Web of Science ID 000088006300006

    View details for PubMedID 10890359

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