Cancer Institute A national cancer institute
designated cancer center

Christopher H. Contag

Publication Details

  • Confocal fluorescence microscope with dual-axis architecture and biaxial postobjective scanning JOURNAL OF BIOMEDICAL OPTICS Wang, T. D., Contag, C. H., Mandella, M. J., Chan, N. Y., Kino, G. S. 2004; 9 (4): 735-742

    Abstract:

    We present a novel confocal microscope that has dual-axis architecture and biaxial postobjective scanning for the collection of fluorescence images from biological specimens. This design uses two low-numerical-aperture lenses to achieve high axial resolution and long working distance, and the scanning mirror located distal to the lenses rotates along the orthogonal axes to produce arc-surface images over a large field of view (FOV). With fiber optic coupling, this microscope can potentially be scaled down to millimeter dimensions via microelectromechanical systems (MEMS) technology. We demonstrate a benchtop prototype with a spatial resolution < or =4.4 microm that collects fluorescence images with a high SNR and a good contrast ratio from specimens expressing GFP. Furthermore, the scanning mechanism produces only small differences in aberrations over the image FOV. These results demonstrate proof of concept of the dual-axis confocal architecture for in vivo molecular and cellular imaging.

    View details for Web of Science ID 000222865800008

    View details for PubMedID 15250760

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