Cancer Institute A national cancer institute
designated cancer center

Lawrence Leung

Publication Details

  • Molecular cloning of nonsecreted endothelial cell-derived lipase isoforms GENOMICS Ishida, T., Zheng, Z., Dichek, H. L., Wang, H. J., Moreno, I., Yang, E., Kundu, R. K., Talbi, S., Hirata, K. I., Leung, L. L., Quertermous, T. 2004; 83 (1): 24-33


    To expand our knowledge of factors involved in lipid metabolism in the blood vessel wall, we have cloned unique molecular isoforms of endothelial cell-derived lipase (EDL) (HGMW-approved symbol/LIPG). One isoform encoded a truncated protein (EDL2a) lacking the first 80 amino acid residues of the previously characterized EDL1a isoform, including the signal peptide. A similar second clone (EDL2b) was identified that lacked not only the first 80 amino acids, but also a 74-amino-acid region that encodes a portion of the lid domain. RT-PCR analysis confirmed expression of EDL2a/2b isoforms in several human tissues and cultured cells, including endothelial cells. Western blot and immunofluorescence studies using stable transfectants revealed that EDL2a and EDL2b were localized in the cytosol, while, EDL1a was secreted into the culture medium. Cell extracts of EDL2a/2b transfectants did not have triglyceride or phospholipase activity. Thus endothelial cells express three EDL isoforms, two of which remain intracellular and do not function as lipases.

    View details for DOI 10.1016/S0888-7543(03)00181-2

    View details for Web of Science ID 000187775700004

    View details for PubMedID 14667806

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