Cancer Institute A national cancer institute
designated cancer center

Christopher H. Contag

Publication Details

  • Advances in vivo bioluminescence imaging of gene expression ANNUAL REVIEW OF BIOMEDICAL ENGINEERING Contag, C. H., Bachmann, M. H. 2002; 4: 235-260


    To advance our understanding of biological processes as they occur in living animals, imaging strategies have been developed and refined that reveal cellular and molecular features of biology and disease in real time. One rapid and accessible technology for in vivo analysis employs internal biological sources of light emitted from luminescent enzymes, luciferases, to label genes and cells. Combining this reporter system with the new generation of charge coupled device (CCD) cameras that detect the light transmitted through the animal's tissues has opened the door to sensitive in vivo measurements of mammalian gene expression in living animals. Here, we review the development and application of this imaging strategy, in vivo bioluminescence imaging (BLI), together with in vivo fluorescence imaging methods, which has enabled the real-time study of immune cell trafficking, of various genetic regulatory elements in transgenic mice, and of in vivo gene transfer. BLI has been combined with fluorescence methods that together offer access to in vivo measurements that were not previously available. Such studies will greatly facilitate the functional analysis of a wide range of genes for their roles in health and disease.

    View details for DOI 10.1146/annurev.bioeng.4.111901.093336

    View details for Web of Science ID 000177827800011

    View details for PubMedID 12117758

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