Cancer Institute A national cancer institute
designated cancer center

Matthew Bogyo

Publication Details

  • Chemical approaches for functionally probing the proteome MOLECULAR & CELLULAR PROTEOMICS Greenbaum, D., Baruch, A., Hayrapetian, L., Darula, Z., Burlingame, A., Medzihradszky, K. F., Bogyo, M. 2002; 1 (1): 60-68


    With the availability of complete genome sequences, emphasis has shifted toward the understanding of protein function. We have developed a functional proteomic methodology that makes use of chemically reactive fluorescent probes to profile and identify enzymes in complex mixtures by virtue of their catalytic activity. This methodology allows a comparison of changes in activity of multiple enzymes under a variety of conditions using a single two-dimensional separation. The probes can also be used to localize active enzymes in intact cells using fluorescence microscopy. Furthermore, the probes enable screens for selective small molecule inhibitors of each enzyme family member within crude lysates or intact cells. Ultimately, this technology allows the rapid identification of potential drug targets and small molecule lead compounds targeted to them.

    View details for DOI 10.1074/mcp.T100003-MCP200

    View details for Web of Science ID 000181445400007

    View details for PubMedID 12096141

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