Cancer Institute A national cancer institute
designated cancer center

Jeffrey Axelrod

Publication Details

  • Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways GENES & DEVELOPMENT Axelrod, J. D., Miller, J. R., Shulman, J. M., MOON, R. T., Perrimon, N. 1998; 12 (16): 2610-2622

    Abstract:

    In Drosophila, planar cell polarity (PCP) signaling is mediated by the receptor Frizzled (Fz) and transduced by Dishevelled (Dsh). Wingless (Wg) signaling also requires Dsh and may utilize DFz2 as a receptor. Using a heterologous system, we show that Dsh is recruited selectively to the membrane by Fz but not DFz2, and this recruitment depends on the DEP domain but not the PDZ domain in Dsh. A mutation in the DEP domain impairs both membrane localization and the function of Dsh in PCP signaling, indicating that translocation is important for function. Further genetic and molecular analyses suggest that conserved domains in Dsh function differently during PCP and Wg signaling, and that divergent intracellular pathways are activated. We propose that Dsh has distinct roles in PCP and Wg signaling. The PCP signal may selectively result in focal Fz activation and asymmetric relocalization of Dsh to the membrane, where Dsh effects cytoskeletal reorganization to orient prehair initiation.

    View details for Web of Science ID 000075604900015

    View details for PubMedID 9716412

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