Cancer Institute A national cancer institute
designated cancer center

Atul Butte

Publication Details

  • TYROSINE KINASE-DEFICIENT MUTANT HUMAN INSULIN-RECEPTORS (MET(1153)-]ILE) OVEREXPRESSED IN TRANSFECTED RAT ADIPOSE-CELLS FAIL TO MEDIATE TRANSLOCATION OF EPITOPE-TAGGED GLUT4 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Quon, M. J., GUERREMILLO, M., Zarnowski, M. J., Butte, A. J., Em, M., Cushman, S. W., Taylor, S. I. 1994; 91 (12): 5587-5591

    Abstract:

    Insulin regulates essential pathways for growth, differentiation, and metabolism in vivo. We report a physiologically relevant system for dissecting the molecular mechanisms of insulin signal transduction related to glucose transport. This is an extension of our recently reported method for transfection of DNA into rat adipose cells in primary culture. In the present work, cDNA coding for GLUT4 with an epitope tag (HA1) in the first exofacial loop is used as a reporter gene so that GLUT4 translocation can be studied exclusively in transfected cells. Insulin stimulates a 4.3-fold recruitment of transfected epitope-tagged GLUT4 to the cell surface. Cells cotransfected with the reporter gene and the human insulin receptor gene show an increase in cell surface GLUT4 in the basal state (no insulin) to levels comparable to those seen with maximal insulin stimulation of cells transfected with the reporter gene alone. In contrast, cells overexpressing a naturally occurring tyrosine kinase-deficient mutant insulin receptor (Met1153-->Ile) show no increase in the basal cell surface GLUT4 and no shift in the insulin dose-response curve relative to cells transfected with the reporter gene alone. These results demonstrate that insulin receptor tyrosine kinase activity is essential in insulin-stimulated glucose transport in adipose cells.

    View details for Web of Science ID A1994NR27500077

    View details for PubMedID 8202531

Stanford Medicine Resources:

Footer Links: