Cancer Institute A national cancer institute
designated cancer center

Ronald Levy, MD

Publication Details

  • FUNCTIONAL IMMUNOGLOBULIN LIGHT CHAIN GENES ARE REPLACED BY ONGOING REARRANGEMENTS OF GERMLINE VK GENES TO DOWNSTREAM JK SEGMENTS IN A MURINE B-CELL LINE JOURNAL OF EXPERIMENTAL MEDICINE Levy, S., Campbell, M. J., Levy, R. 1989; 170 (1): 1-13

    Abstract:

    A murine B cell lymphoma (38C13) was subjected to immunoselection with mAbs directed against the idiotypic determinants of its cell surface Ig. Variants emerged with altered Ig receptors containing identical heavy chains but different light chains. The functional light chain genes in these variants were composed of V kappa segments drawn from the V kappa Ox-1 family, which had replaced the V kappa gene expressed by the parental tumor by rearranging to downstream J kappa segments. Rearrangement at the kappa locus continued to occur spontaneously, giving rise to secondary and tertiary variants at a rate of 1.9 x 10(-4) per cell per generation. Variants were isolated that had ceased production of surface Ig but went on to rearrange again and to become surface Ig+. The Ig- state may be an intermediate step providing a stimulus for continued rearrangement. This process provides an additional mechanism for generating diversity within B cell clones and expands the use of the available repertoire of Ig genes.

    View details for Web of Science ID A1989AE68500001

    View details for PubMedID 2501443

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