Cancer Institute A national cancer institute
designated cancer center

Roeland Nusse

Publication Details

  • A Localized Wnt Signal Orients Asymmetric Stem Cell Division in Vitro SCIENCE Habib, S. J., Chen, B., Tsai, F., Anastassiadis, K., Meyer, T., Betzig, E., Nusse, R. 2013; 339 (6126): 1445-1448


    Developmental signals such as Wnts are often presented to cells in an oriented manner. To examine the consequences of local Wnt signaling, we immobilized Wnt proteins on beads and introduced them to embryonic stem cells in culture. At the single-cell level, the Wnt-bead induced asymmetric distribution of Wnt-?-catenin signaling components, oriented the plane of mitotic division, and directed asymmetric inheritance of centrosomes. Before cytokinesis was completed, the Wnt-proximal daughter cell expressed high levels of nuclear ?-catenin and pluripotency genes, whereas the distal daughter cell acquired hallmarks of differentiation. We suggest that a spatially restricted Wnt signal induces an oriented cell division that generates distinct cell fates at predictable positions relative to the Wnt source.

    View details for DOI 10.1126/science.1231077

    View details for Web of Science ID 000316740700046

    View details for PubMedID 23520113

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