Cancer Institute A national cancer institute
designated cancer center

Manish J. Butte, MD PhD

Publication Details

  • Real-time GPU-based 3D Deconvolution OPTICS EXPRESS Bruce, M. A., Butte, M. J. 2013; 21 (4): 4766-4773


    Confocal microscopy is an oft-used technique in biology. Deconvolution of 3D images reduces blurring from out-of-focus light and enables quantitative analyses, but existing software for deconvolution is slow and expensive. We present a parallelized software method that runs within ImageJ and deconvolves 3D images ~100 times faster than conventional software (few seconds per image) by running on a low-cost graphics processor board (GPU). We demonstrate the utility of this software by analyzing microclusters of T cell receptors in the immunological synapse of a CD4 + T cell and dendritic cell. This software provides a low-cost and rapid way to improve the accuracy of 3D microscopic images obtained by any method.

    View details for Web of Science ID 000315992600083

    View details for PubMedID 23482010

Stanford Medicine Resources:

Footer Links: