Cancer Institute A national cancer institute
designated cancer center

Howard Y. Chang

Publication Details

  • RNA SHAPE analysis in living cells NATURE CHEMICAL BIOLOGY Spitale, R. C., Crisalli, P., Flynn, R. A., Torre, E. A., Kool, E. T., Chang, H. Y. 2013; 9 (1): 18-?

    Abstract:

    RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) in living cells. RNA structures in human, mouse, fly, yeast and bacterial cells are read out at single-nucleotide resolution, revealing tertiary contacts and RNA-protein interactions.

    View details for DOI 10.1038/NCHEMBIO.1131

    View details for Web of Science ID 000312484200007

    View details for PubMedID 23178934

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