Cancer Institute A national cancer institute
designated cancer center

Michael Link

Publication Details

  • DOWN-REGULATED C-MYB EXPRESSION INHIBITS DNA-SYNTHESIS OF T-LEUKEMIA CELLS IN MOST PATIENTS CANCER RESEARCH Venturelli, D., MARIANO, M. T., Szczylik, C., Valtieri, M., Lange, B., Crist, W., Link, M., Calabretta, B. 1990; 50 (22): 7371-7375

    Abstract:

    We have investigated the functional relevance of c-myb expression for DNA synthesis in patients' T-leukemia cells. [3H]Thymidine incorporation assays of 32 patients' leukemia cells exposed in vitro to c-myb sense or antisense oligodeoxynucleotides served to define two groups of patients: a responder group whose leukemia cells showed 2- to 16-fold lower levels of [3H]thymidine incorporation in c-myb antisense-treated cultures than in c-myb sense-treated cultures (20 patients) and a nonresponder group whose cells showed comparable [3H]thymidine incorporation levels in either c-myb sense- or antisense-treated cultures (12 patients). Down-regulation of c-myb mRNA levels in cells exposed to c-myb antisense oligodeoxynucleotides was comparable in both groups of patients, indicating that differential sensitivity to c-myb antisense oligodeoxynucleotides was not due to differential uptake of these oligodeoxynucleotides. DNA polymerase alpha mRNA levels were down-regulated in cells from the responders but were unaffected in the nonresponder group. These results suggest that c-myb is required for DNA synthesis in cells of many but not all T-leukemia patients and that leukemia cells in which DNA synthesis is not inhibited despite down-regulation of c-myb expression may have undergone some genetic change(s) that obviate(s) the requirement for myb protein.

    View details for Web of Science ID A1990EH00600045

    View details for PubMedID 2224864

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