Cancer Institute A national cancer institute
designated cancer center

Roeland Nusse

Publication Details

  • Secreted Wingless-interacting molecule (Swim) promotes long-range signaling by maintaining Wingless solubility PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Mulligan, K. A., Fuerer, C., Ching, W., Fish, M., Willert, K., Nusse, R. 2012; 109 (2): 370-377

    Abstract:

    Lipid-modified Wnt/Wingless (Wg) proteins can signal to their target cells in a short- or long-range manner. How these hydrophobic proteins travel through the extracellular environment remains an outstanding question. Here, we report on a Wg binding protein, Secreted Wg-interacting molecule (Swim), that facilitates Wg diffusion through the extracellular matrix. Swim, a putative member of the Lipocalin family of extracellular transport proteins, binds to Wg with nanomolar affinity in a lipid-dependent manner. In quantitative signaling assays, Swim is sufficient to maintain the solubility and activity of purified Wg. In Drosophila, swim RNAi phenotypes resemble wg loss-of-function phenotypes in long-range signaling. We propose that Swim is a cofactor that promotes long-range Wg signaling in vivo by maintaining the solubility of Wg.

    View details for DOI 10.1073/pnas.1119197109

    View details for Web of Science ID 000298950200014

    View details for PubMedID 22203956

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