Cancer Institute A national cancer institute
designated cancer center

Roeland Nusse

Publication Details

  • A Suppressor/Enhancer Screen in Drosophila Reveals a Role for Wnt-Mediated Lipid Metabolism in Primordial Germ Cell Migration PLOS ONE McElwain, M. A., Ko, D. C., Gordon, M. D., Fyrst, H., Saba, J. D., Nusse, R. 2011; 6 (11)

    Abstract:

    Wnt proteins comprise a large family of secreted ligands implicated in a wide variety of biological roles. WntD has previously been shown to inhibit the nuclear accumulation of Dorsal/NF-?B protein during embryonic dorsal/ventral patterning and the adult innate immune response, independent of the well-studied Armadillo/?-catenin pathway. In this paper, we present a novel phenotype for WntD mutant embryos, suggesting that this gene is involved in migration of primordial germ cells (PGC) to the embryonic gonad. Additionally, we describe a genetic suppressor/enhancer screen aimed at identifying genes required for WntD signal transduction, based on the previous observation that maternal overexpression of WntD results in lethally dorsalized embryos. Using an algorithm to narrow down our hits from the screen, we found two novel WntD signaling components: Fz4, a member of the Frizzled family, and the Drosophila Ceramide Kinase homolog, Dcerk. We show here that Dcerk and Dmulk (Drosophila Multi-substrate lipid kinase) redundantly mediate PGC migration. Our data are consistent with a model in which the activity of lipid phosphate phosphatases shapes a concentration gradient of ceramide-1-phosphate (C1P), the product of Dcerk, allowing proper PGC migration.

    View details for DOI 10.1371/journal.pone.0026993

    View details for Web of Science ID 000297150900042

    View details for PubMedID 22069480

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