Cancer Institute A national cancer institute
designated cancer center

James Spudich

Publication Details

  • Proteomics approach to study the functions of Drosophila myosin VI through identification of multiple cargo-binding proteins PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Finan, D., Hartman, A., Spudich, J. A. 2011; 108 (14): 5566-5571


    Myosin VI is a molecular motor implicated in many processes, and it likely associates with a variety of cargoes that specify its functions. Although it is critical to Drosophila development, little is known about its cellular roles. To reveal its involvement in specific pathways, we sought to identify the binding partners of Drosophila myosin VI. We used affinity chromatography and mass spectrometry to discover interacting proteins, which we tested for direct binding. Using this approach, we found that the microtubule-associated protein Cornetto bound myosin VI, and we demonstrated a role for both in secretion of the lipidated morphogen Hedgehog. We also identified a number of other binding proteins, and further characterization of their interactions with myosin VI will advance our understanding of the roles of these complexes in cellular and developmental processes. Thus, our method has provided us the means to gain valuable insight into the multifaceted roles of a motor protein in vivo.

    View details for DOI 10.1073/pnas.1101415108

    View details for Web of Science ID 000289265300020

    View details for PubMedID 21368190

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