Cancer Institute A national cancer institute
designated cancer center

Matthew Bogyo

Publication Details

  • Development of Activity-Based Probes for Cathepsin X ACS CHEMICAL BIOLOGY Paulick, M. G., Bogyo, M. 2011; 6 (6): 563-572

    Abstract:

    Cathepsin X is a lysosomal cysteine protease that functions as a carboxypeptidase with broad substrate specificity. Cathepsin X was discovered only recently, and its physiological roles are still not well understood. A number of studies suggest that cathepsin X may be involved in a variety of biological processes, including cancer, aging and degenerative conditions of the brain, inflammation, and cellular communication. Here we present the synthesis and characterization of several activity-based probes (ABPs) that target active cathepsin X. These ABPs were used to label cathepsin X in complex lysates, whole cells, and in vivo. Furthermore, we have developed a method for selectively labeling and visualizing active cathepsin X in vitro and in vivo. Overall, the probes developed in this study are valuable tools for the study of cathepsin X function.

    View details for DOI 10.1021/cb100392r

    View details for Web of Science ID 000291896400006

    View details for PubMedID 21322635

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