James Spudich
Publications
- Cell-intrinsic functional effects of the α-cardiac myosin Arg-403-Gln mutation in familial hypertrophic cardiomyopathy. Biophys J. 2012; (12): 2782-90
- The myosin superfamily at a glance. J Cell Sci. 2012; (Pt 7): 1627-32
- Biochemistry. Molecular motors, beauty in complexity. Science. 2011; (6021): 1143-4
- Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI. Biophys J. 2011; (2): 430-9
- Molecular motors: forty years of interdisciplinary research. Mol Biol Cell. 2011; (21): 3936-9
- Principles of unconventional myosin function and targeting. Annu Rev Cell Dev Biol. 2011: 133-55
- Proteomics approach to study the functions of Drosophila myosin VI through identification of multiple cargo-binding proteins. Proc Natl Acad Sci U S A. 2011; (14): 5566-71
- Robust mechanosensing and tension generation by myosin VI. J Mol Biol. 2011; (1): 105-12
- Systematic control of protein interaction using a modular ER/K α-helix linker. Proc Natl Acad Sci U S A. 2011; (51): 20467-72
- Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing. Proc Natl Acad Sci U S A. 2010; (17): 7746-50
- Erratum: Insights into Human β-Cardiac Myosin Function from Single Molecule and Single Cell Studies. J Cardiovasc Transl Res. 2010
- Helicity of short E-R/K peptides. Protein Sci. 2010; (10): 2001-5
- Myosin VI: an innovative motor that challenged the swinging lever arm hypothesis. Nat Rev Mol Cell Biol. 2010; (2): 128-37
- Single-molecule dual-beam optical trap analysis of protein structure and function. Methods Enzymol. 2010: 321-75
- Combining single-molecule optical trapping and small-angle x-ray scattering measurements to compute the persistence length of a protein ER/K alpha-helix. Biophys J. 2009; (11): 2993-9
- Coupled myosin VI motors facilitate unidirectional movement on an F-actin network. J Cell Biol. 2009; (1): 53-60
- Engineered myosin VI motors reveal minimal structural determinants of directionality and processivity. J Mol Biol. 2009; (4): 862-7
- Insights into human beta-cardiac myosin function from single molecule and single cell studies. J Cardiovasc Transl Res. 2009; (4): 426-40
- Dynamic charge interactions create surprising rigidity in the ER/K alpha-helical protein motif. Proc Natl Acad Sci U S A. 2008; (36): 13356-61
- Dynamic organization of gene loci and transcription compartments in the cell nucleus. Biophys J. 2008; (11): 5003-4
- Long single alpha-helical tail domains bridge the gap between structure and function of myosin VI. Nat Struct Mol Biol. 2008; (6): 591-7
- Molecular motors: a surprising twist in myosin VI translocation. Curr Biol. 2008; (2): R68-70
- Dynamics of the unbound head during myosin V processive translocation. Nat Struct Mol Biol. 2007; (3): 246-8
- Extending the absorbing boundary method to fit dwell-time distributions of molecular motors with complex kinetic pathways. Proc Natl Acad Sci U S A. 2007; (9): 3171-6
- Identification of a minimal myosin Va binding site within an intrinsically unstructured domain of melanophilin. J Biol Chem. 2007; (29): 21518-28
- Precise positioning of myosin VI on endocytic vesicles in vivo. PLoS Biol. 2007; (8): e210
- Predicting allosteric communication in myosin via a pathway of conserved residues. J Mol Biol. 2007; (5): 1361-73
- The localization of inner centromeric protein (INCENP) at the cleavage furrow is dependent on Kif12 and involves interactions of the N terminus of INCENP with the actin cytoskeleton. Mol Biol Cell. 2007; (9): 3366-74
- The power stroke of myosin VI and the basis of reverse directionality. Proc Natl Acad Sci U S A. 2007; (3): 772-7
- A non-Gaussian distribution quantifies distances measured with fluorescence localization techniques. Biophys J. 2006; (2): 668-71
- Molecular motors take tension in stride. Cell. 2006; (2): 242-4
- Rho kinase's role in myosin recruitment to the equatorial cortex of mitotic Drosophila S2 cells is for myosin regulatory light chain phosphorylation. PLoS One. 2006: e131
- A flexible domain is essential for the large step size and processivity of myosin VI. Mol Cell. 2005; (4): 603-9
- A force-dependent state controls the coordination of processive myosin V. Proc Natl Acad Sci U S A. 2005; (39): 13873-8
- Distinct pathways control recruitment and maintenance of myosin II at the cleavage furrow during cytokinesis. Proc Natl Acad Sci U S A. 2005; (38): 13473-8
- Single molecule high-resolution colocalization of Cy3 and Cy5 attached to macromolecules measures intramolecular distances through time. Proc Natl Acad Sci U S A. 2005; (5): 1419-23
- A mitotic kinesin-like protein required for normal karyokinesis, myosin localization to the furrow, and cytokinesis in Dictyostelium. Proc Natl Acad Sci U S A. 2004; (47): 16519-24
- Dictyostelium myosin bipolar thick filament formation: importance of charge and specific domains of the myosin rod. PLoS Biol. 2004; (11): e356
- Myosin VI walks hand-over-hand along actin. Nat Struct Mol Biol. 2004; (9): 884-7
- The mechanism of myosin VI translocation and its load-induced anchoring. Cell. 2004; (5): 737-49
- Two important polymers cross paths. Proc Natl Acad Sci U S A. 2004; (45): 15825-6
- Building and using optical traps to study properties of molecular motors. Methods Enzymol. 2003: 112-33
- Structure of an F-actin trimer disrupted by gelsolin and implications for the mechanism of severing. J Biol Chem. 2003; (2): 1229-38
- Differential localization in cells of myosin II heavy chain kinases during cytokinesis and polarized migration. BMC Cell Biol. 2002: 19
- Dynacortin is a novel actin bundling protein that localizes to dynamic actin structures. J Biol Chem. 2002; (11): 9088-95
- Quantitation of the distribution and flux of myosin-II during cytokinesis. BMC Cell Biol. 2002: 4
- Role of the lever arm in the processive stepping of myosin V. Proc Natl Acad Sci U S A. 2002; (22): 14159-64
- A myosin II mutation uncouples ATPase activity from motility and shortens step size. Nat Cell Biol. 2001; (3): 311-5
- Myosin VI is a processive motor with a large step size. Proc Natl Acad Sci U S A. 2001; (24): 13655-9
- The myosin relay helix to converter interface remains intact throughout the actomyosin ATPase cycle. J Biol Chem. 2001; (22): 19491-4
- The myosin swinging cross-bridge model. Nat Rev Mol Cell Biol. 2001; (5): 387-92
- A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin. Cell. 2000; (5): 683-94
- Dynacortin, a genetic link between equatorial contractility and global shape control discovered by library complementation of a Dictyostelium discoideum cytokinesis mutant. J Cell Biol. 2000; (4): 823-38
- In vitro assays of processive myosin motors. Methods. 2000; (4): 373-81
- Mutational analysis of phosphorylation sites in the Dictyostelium myosin II tail: disruption of myosin function by a single charge change. FEBS Lett. 2000; (2-3): 267-72
- Myosin-V stepping kinetics: a molecular model for processivity. Proc Natl Acad Sci U S A. 2000; (17): 9482-6
- Towards a molecular understanding of cytokinesis. Trends Cell Biol. 2000; (6): 228-37
- Variable surface loops and myosin activity: accessories to a motor. J Muscle Res Cell Motil. 2000; (2): 139-51
- A structural model for phosphorylation control of Dictyostelium myosin II thick filament assembly. J Cell Biol. 1999; (5): 1039-48
- Biomechanics, one molecule at a time. J Biol Chem. 1999; (21): 14517-20
- Myosin-V is a processive actin-based motor. Nature. 1999; (6744): 590-3
- Single-molecule biomechanics with optical methods. Science. 1999; (5408): 1689-95
- Specialized conservation of surface loops of myosin: evidence that loops are involved in determining functional characteristics. J Mol Biol. 1999; (1): 173-85
- The sequence of the myosin 50-20K loop affects Myosin's affinity for actin throughout the actin-myosin ATPase cycle and its maximum ATPase activity. Biochemistry. 1999; (12): 3785-92
- Conditional loss-of-myosin-II-function mutants reveal a position in the tail that is critical for filament nucleation. Mol Cell. 1998; (7): 1043-50
- Dictyostelium myosin 25-50K loop substitutions specifically affect ADP release rates. Biochemistry. 1998; (19): 6738-44
- Kinetic characterization of myosin head fragments with long-lived myosin.ATP states. Biochemistry. 1998; (27): 9679-87
- MLCK-A, an unconventional myosin light chain kinase from Dictyostelium, is activated by a cGMP-dependent pathway. Proc Natl Acad Sci U S A. 1998; (22): 13000-5
- Myosin II localization during cytokinesis occurs by a mechanism that does not require its motor domain. Proc Natl Acad Sci U S A. 1998; (23): 13652-7
- Nucleotide-dependent conformational change near the fulcrum region in Dictyostelium myosin II. Proc Natl Acad Sci U S A. 1998; (22): 12844-7
- Reflections of a lucid dreamer: optical trap design considerations. Methods Cell Biol. 1998: 47-69
- Single molecule biochemistry using optical tweezers. FEBS Lett. 1998; (1-2): 23-7
- Use of optical traps in single-molecule study of nonprocessive biological motors. Methods Enzymol. 1998: 436-59
- Bioreactive self-assembled monolayers on hydrogen-passivated Si(111) as a new class of atomically flat substrates for biological scanning probe microscopy. J Struct Biol. 1997; (2): 189-201
- Detection of single-molecule interactions using correlated thermal diffusion. Proc Natl Acad Sci U S A. 1997; (15): 7927-31
- Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesis in live cells. Mol Biol Cell. 1997; (12): 2605-15
- On the role of myosin-II in cytokinesis: division of Dictyostelium cells under adhesive and nonadhesive conditions. Mol Biol Cell. 1997; (12): 2617-29
- Phenotypically selected mutations in myosin's actin binding domain demonstrate intermolecular contacts important for motor function. Biochemistry. 1997; (28): 8465-73
- Reversible, site-specific immobilization of polyarginine-tagged fusion proteins on mica surfaces. FEBS Lett. 1997; (2): 233-8
- Activation of Dictyostelium myosin light chain kinase A by phosphorylation of Thr166. EMBO J. 1996; (22): 6075-83
- Cold-sensitive mutations of Dictyostelium myosin heavy chain highlight functional domains of the myosin motor. Genetics. 1996; (2): 801-10
- Myosin dynamics in live Dictyostelium cells. Proc Natl Acad Sci U S A. 1996; (1): 443-6
- Myosin light chain kinase (MLCK) gene disruption in Dictyostelium: a role for MLCK-A in cytokinesis and evidence for multiple MLCKs. Proc Natl Acad Sci U S A. 1996; (22): 12321-6
- Structure-function analysis of the motor domain of myosin. Annu Rev Cell Dev Biol. 1996: 543-73
- Structure-function studies of the myosin motor domain: importance of the 50-kDa cleft. Mol Biol Cell. 1996; (7): 1123-36
- Synthetic lethality screen identifies a novel yeast myosin I gene (MYO5): myosin I proteins are required for polarization of the actin cytoskeleton. J Cell Biol. 1996; (6): 1277-91
- The Dictyostelium dual-specificity kinase splA is essential for spore differentiation. Development. 1996; (10): 3295-305
- A novel positive selection for identifying cold-sensitive myosin II mutants in Dictyostelium. Genetics. 1995; (2): 505-15
- Characterization of single actin-myosin interactions. Biophys J. 1995; (4 Suppl): 291S-296S; discussion 296S-297S
- Identification and molecular characterization of a yeast myosin I. Cell Motil Cytoskeleton. 1995; (1): 73-84
- Myosin motor function: structural and mutagenic approaches. Curr Opin Cell Biol. 1995; (1): 89-93
- Myosin structure and function. Cold Spring Harb Symp Quant Biol. 1995: 783-91
- Myosin structure/function: a combined mutagenesis-crystallography approach. Curr Opin Struct Biol. 1995; (2): 181-6
- Enzymatic activities correlate with chimaeric substitutions at the actin-binding face of myosin. Nature. 1994; (6471): 567-9
- How molecular motors work. Nature. 1994; (6506): 515-8
- Molecular genetic truncation analysis of filament assembly and phosphorylation domains of Dictyostelium myosin heavy chain. J Cell Sci. 1994: 2875-86
- Role of highly conserved lysine 130 of myosin motor domain. In vivo and in vitro characterization of site specifically mutated myosin. J Biol Chem. 1994; (29): 18773-80
- Single myosin molecule mechanics: piconewton forces and nanometre steps. Nature. 1994; (6467): 113-9
- Stage-specific requirement for myosin II during Dictyostelium development. Development. 1994; (9): 2651-60
- A functional recombinant myosin II lacking a regulatory light chain-binding site. Science. 1993; (5141): 1867-70
- Dictyostelium myosin heavy chain phosphorylation sites regulate myosin filament assembly and localization in vivo. Cell. 1993; (2): 363-71
- In vitro methods for measuring force and velocity of the actin-myosin interaction using purified proteins. Methods Cell Biol. 1993: 1-21
- Molecular evolution of the myosin family: relationships derived from comparisons of amino acid sequences. Proc Natl Acad Sci U S A. 1993; (2): 659-63
- The unconventional myosin encoded by the myoA gene plays a role in Dictyostelium motility. Mol Biol Cell. 1993; (2): 233-46
- A Dictyostelium myosin II lacking a proximal 58-kDa portion of the tail is functional in vitro and in vivo. Mol Biol Cell. 1992; (12): 1455-62
- Control of nonmuscle myosins by phosphorylation. Annu Rev Biochem. 1992: 721-59
- Membrane-bound Dictyostelium myosin heavy chain kinase: a developmentally regulated substrate-specific member of the protein kinase C family. Proc Natl Acad Sci U S A. 1992; (13): 5877-81
- Yeast actin filaments display ATP-dependent sliding movement over surfaces coated with rabbit muscle myosin. Proc Natl Acad Sci U S A. 1992; (10): 4466-70
- An approach to reconstituting motility of single myosin molecules. J Cell Sci Suppl. 1991: 129-33
- Characterization and bacterial expression of the Dictyostelium myosin light chain kinase cDNA. Identification of an autoinhibitory domain. J Biol Chem. 1991; (24): 16044-9
- Molecular genetic approaches to the cytoskeleton in Dictyostelium. Curr Opin Genet Dev. 1991; (3): 378-82
- Molecular genetics of cell migration: Dictyostelium as a model system. Trends Genet. 1991; (5): 161-6
- Quantized velocities at low myosin densities in an in vitro motility assay. Nature. 1991; (6333): 307-11
- Spatial and temporal control of nonmuscle myosin localization: identification of a domain that is necessary for myosin filament disassembly in vivo. J Cell Biol. 1991; (4): 677-88
- Complementation of myosin null mutants in Dictyostelium discoideum by direct functional selection. Dev Biol. 1990; (2): 359-67
- Developmentally regulated protein-tyrosine kinase genes in Dictyostelium discoideum. Mol Cell Biol. 1990; (7): 3578-83
- Dictyostelium myosin light chain kinase. Purification and characterization. J Biol Chem. 1990; (23): 13818-24
- Expression of Dictyostelium myosin tail segments in Escherichia coli: domains required for assembly and phosphorylation. J Cell Biol. 1990; (1): 63-70
- Genetically engineered truncated myosin in Dictyostelium: the carboxyl-terminal regulatory domain is not required for the developmental cycle. Proc Natl Acad Sci U S A. 1990; (20): 8110-4
- Molecular genetics: a key to the cytoskeleton's closet. Curr Opin Cell Biol. 1990; (1): 116-20
- Myosin step size. Estimation from slow sliding movement of actin over low densities of heavy meromyosin. J Mol Biol. 1990; (3): 699-710
- Purification of a functional recombinant myosin fragment from Dictyostelium discoideum. Ann N Y Acad Sci. 1990: 147-55
- The myosin step size: measurement of the unit displacement per ATP hydrolyzed in an in vitro assay. Proc Natl Acad Sci U S A. 1990; (18): 7130-4
- Bidirectional movement of actin filaments along tracks of myosin heads. Nature. 1989; (6238): 154-6
- Capping of surface receptors and concomitant cortical tension are generated by conventional myosin. Nature. 1989; (6242): 549-51
- Expression and characterization of a functional myosin head fragment in Dictyostelium discoideum. Science. 1989; (4930): 656-8
- Gene replacement in Dictyostelium: generation of myosin null mutants. EMBO J. 1989; (3): 923-32
- Hygromycin resistance as a selectable marker in Dictyostelium discoideum. Mol Cell Biol. 1989; (5): 1965-8
- In pursuit of myosin function. Cell Regul. 1989; (1): 1-11
- Intermolecular versus intramolecular interactions of Dictyostelium myosin: possible regulation by heavy chain phosphorylation. J Cell Biol. 1989; (1): 203-10
- Multiple actin-based motor genes in Dictyostelium. Cell Regul. 1989; (1): 55-63
- Myosin heavy chain kinase from developed Dictyostelium cells. Purification and characterization. J Biol Chem. 1989; (25): 15144-50
- Codon preference in Dictyostelium discoideum. Nucleic Acids Res. 1988; (14A): 6617-35
- Expression in Escherichia coli of a functional Dictyostelium myosin tail fragment. J Cell Biol. 1987; (6 Pt 2): 2999-3005
- Myosin structure and function in cell motility. Annu Rev Cell Biol. 1987: 379-421
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