James K. Chen

Publications

• Functional inhibition of UQCRB suppresses angiogenesis in zebrafish. Cho YS, Jung HJ, Seok SH, Payumo AY, Chen JK, Kwon HJ. Biochem Biophys Res Commun. 2013; 433 (4): 396-400
• Cyclic caged morpholinos: conformationally gated probes of embryonic gene function. Yamazoe S, Shestopalov IA, Provost E, Leach SD, Chen JK. Angew Chem Int Ed Engl. 2012; 51 (28): 6908-11
• Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein. Firestone AJ, Weinger JS, Maldonado M, Barlan K, Langston LD, O'Donnell M, Gelfand VI, Kapoor TM, Chen JK. Nature. 2012; 484 (7392): 125-9
• Spatiotemporal resolution of the Ntla transcriptome in axial mesoderm development. Shestopalov IA, Pitt CL, Chen JK. Nat Chem Biol. 2012; 8 (3): 270-6
• The BAH domain of ORC1 links H4K20me2 to DNA replication licensing and Meier-Gorlin syndrome. Kuo AJ, Song J, Cheung P, Ishibe-Murakami S, Yamazoe S, Chen JK, Patel DJ, Gozani O. Nature. 2012; 484 (7392): 115-9
• A crucial requirement for Hedgehog signaling in small cell lung cancer. Park KS, Martelotto LG, Peifer M, Sos ML, Karnezis AN, Mahjoub MR, Bernard K, Conklin JF, Szczepny A, Yuan J, Guo R, Ospina B, Falzon J, Bennett S, Brown TJ, Markovic A, Devereux WL, Ocasio CA, Chen JK, Stearns T, Thomas RK, Dorsch M, Buonamici S, Watkins DN, Peacock CD, Sage J. Nat Med. 2011; 17 (11): 1504-8
• A small-molecule smoothened agonist prevents glucocorticoid-induced neonatal cerebellar injury. Heine VM, Griveau A, Chapin C, Ballard PL, Chen JK, Rowitch DH. Sci Transl Med. 2011; 3 (105): 105ra104
• Chemical 'Jekyll and Hyde's: small-molecule inhibitors of developmental signaling pathways. Sakata T, Chen JK. Chem Soc Rev. 2011; 40 (8): 4318-31
• Hedgehog and retinoic acid signaling cooperate to promote motoneurogenesis in zebrafish. Mich JK, Chen JK. Development. 2011; 138 (23): 5113-9
• Lineage labeling of zebrafish cells with laser uncagable fluorescein dextran. Clanton JA, Shestopalov I, Chen JK, Gamse JT. J Vis Exp. 2011; (50)
• Neuropilins are positive regulators of Hedgehog signal transduction. Hillman RT, Feng BY, Ni J, Woo WM, Milenkovic L, Hayden Gephart MG, Teruel MN, Oro AE, Chen JK, Scott MP. Genes Dev. 2011; 25 (22): 2333-46
• Roles of Hedgehog pathway components and retinoic acid signalling in specifying zebrafish ventral spinal cord neurons. England S, Batista MF, Mich JK, Chen JK, Lewis KE. Development. 2011; 138 (23): 5121-34
• Spatiotemporal control of embryonic gene expression using caged morpholinos. Shestopalov IA, Chen JK. Methods Cell Biol. 2011: 104 151-72
• Controlling destiny through chemistry: small-molecule regulators of cell fate. Firestone AJ, Chen JK. ACS Chem Biol. 2010; 5 (1): 15-34
• Oligonucleotide-based tools for studying zebrafish development. Shestopalov IA, Chen JK. Zebrafish. 2010; 7 (1): 31-40
• Synthetic strategies for studying embryonic development. Ouyang X, Chen JK. Chem Biol. 2010; 17 (6): 590-606
• A small molecule that binds Hedgehog and blocks its signaling in human cells. Stanton BZ, Peng LF, Maloof N, Nakai K, Wang X, Duffner JL, Taveras KM, Hyman JM, Lee SW, Koehler AN, Chen JK, Fox JL, Mandinova A, Schreiber SL. Nat Chem Biol. 2009; 5 (3): 154-6
• Converse conformational control of smoothened activity by structurally related small molecules. Yang H, Xiang J, Wang N, Zhao Y, Hyman J, Li S, Jiang J, Chen JK, Yang Z, Lin S. J Biol Chem. 2009; 284 (31): 20876-84
• Germ cell migration in zebrafish is cyclopamine-sensitive but Smoothened-independent. Mich JK, Blaser H, Thomas NA, Firestone AJ, Yelon D, Raz E, Chen JK. Dev Biol. 2009; 328 (2): 342-54
• Small-molecule inhibitors reveal multiple strategies for Hedgehog pathway blockade. Hyman JM, Firestone AJ, Heine VM, Zhao Y, Ocasio CA, Han K, Sun M, Rack PG, Sinha S, Wu JJ, Solow-Cordero DE, Jiang J, Rowitch DH, Chen JK. Proc Natl Acad Sci U S A. 2009; 106 (33): 14132-7
• The imidazopyridine derivative JK184 reveals dual roles for microtubules in Hedgehog signaling. Cupido T, Rack PG, Firestone AJ, Hyman JM, Han K, Sinha S, Ocasio CA, Chen JK. Angew Chem Int Ed Engl. 2009; 48 (13): 2321-4
• Versatile synthesis and rational design of caged morpholinos. Ouyang X, Shestopalov IA, Sinha S, Zheng G, Pitt CL, Li WH, Olson AJ, Chen JK. J Am Chem Soc. 2009; 131 (37): 13255-69
• Chemical technologies for probing embryonic development. Shestopalov IA, Chen JK. Chem Soc Rev. 2008; 37 (7): 1294-307
• Gene regulation technologies in zebrafish. Esengil H, Chen JK. Mol Biosyst. 2008; 4 (4): 300-8
• Targeted and Conditional Gene Expression Workshop, 8th International Conference on Zebrafish Development and Genetics. Chen JK, Hurlstone AF. Zebrafish. 2008; 5 (3): 193-195
• The decoupling of Smoothened from Galphai proteins has little effect on Gli3 protein processing and Hedgehog-regulated chick neural tube patterning. Low WC, Wang C, Pan Y, Huang XY, Chen JK, Wang B. Dev Biol. 2008; 321 (1): 188-96
• Light-controlled gene silencing in zebrafish embryos. Shestopalov IA, Sinha S, Chen JK. Nat Chem Biol. 2007; 3 (10): 650-1
• Small-molecule regulation of zebrafish gene expression. Esengil H, Chang V, Mich JK, Chen JK. Nat Chem Biol. 2007; 3 (3): 154-5
• Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Sinha S, Chen JK. Nat Chem Biol. 2006; 2 (1): 29-30
• Smoothened signal transduction is promoted by G protein-coupled receptor kinase 2. Meloni AR, Fralish GB, Kelly P, Salahpour A, Chen JK, Wechsler-Reya RJ, Lefkowitz RJ, Caron MG. Mol Cell Biol. 2006; 26 (20): 7550-60
• Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2. Chen W, Ren XR, Nelson CD, Barak LS, Chen JK, Beachy PA, de Sauvage F, Lefkowitz RJ. Science. 2004; 306 (5705): 2257-60
• Inhibition of Hedgehog signaling by direct binding of cyclopamine to Smoothened. Chen JK, Taipale J, Cooper MK, Beachy PA. Genes Dev. 2002; 16 (21): 2743-8
• Medulloblastoma growth inhibition by hedgehog pathway blockade. Berman DM, Karhadkar SS, Hallahan AR, Pritchard JI, Eberhart CG, Watkins DN, Chen JK, Cooper MK, Taipale J, Olson JM, Beachy PA. Science. 2002; 297 (5586): 1559-61
• Small molecule modulation of Smoothened activity. Chen JK, Taipale J, Young KE, Maiti T, Beachy PA. Proc Natl Acad Sci U S A. 2002; 99 (22): 14071-6
• Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine. Taipale J, Chen JK, Cooper MK, Wang B, Mann RK, Milenkovic L, Scott MP, Beachy PA. Nature. 2000; 406 (6799): 1005-9