Annelise E. Barron
Publication Details
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Comb-like copolymers as self-coating, low-viscosity and high-resolution matrices for DNA sequencing.
Electrophoresis. 2002; (10): 1441-9
Comb-like copolymers with a polyacrylamide backbone and poly(N,N-dimethylacrylamide) grafts were prepared, as a way to combine the superior sieving properties of polyacrylamide with the self-coating properties of polydimethylacrylamide. These matrices function well in the absence of a capillary coating, and achieve separation performances for single-stranded DNA that are comparable to those of state-of-the-art long-chain linear polyacrylamide. Structural parameters such as the grafting density and the polymer molecular mass were varied, and good performance appears to be achieved with a relatively large range of parameters. Surprisingly, excellent separation is achieved even with matrices that have a viscosity as low as 200 mPa/s. A discussion of the physics underlying this behavior is provided.
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