Patrick O. Brown
Publication Details
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Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions.
Genome Biol. 2001; (2): RESEARCH0004
We have developed and tested a method for printing protein microarrays and using these microarrays in a comparative fluorescence assay to measure the abundance of many specific proteins in complex solutions. A robotic device was used to print hundreds of specific antibody or antigen solutions in an array on the surface of derivatized microscope slides. Two complex protein samples, one serving as a standard for comparative quantitation, the other representing an experimental sample in which the protein quantities were to be measured, were labeled by covalent attachment of spectrally resolvable fluorescent dyes.
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